NOTCH3-targeted antibody drug conjugates regress tumors by inducing apoptosis in receptor cells and thru transendocytosis into ligand cells
Aberrant NOTCH3 signaling and overexpression is oncogenic, related with most cancers stem cells and drug resistance, but therapeutic concentrating on stays elusive. Right here, we develop NOTCH3-targeted antibody drug conjugates (NOTCH3-ADCs) by bioconjugation of an auristatin microtubule inhibitor by a protease cleavable linker to 2 antibodies with differential talents to inhibit signaling. The signaling inhibitory antibody quickly induces ligand-independent receptor clustering and internalization by each caveolin and clathrin-mediated pathways.
The non-inhibitory antibody additionally effectively endocytoses through clathrin with out inducing receptor clustering however with slower lysosomal co-localization kinetics. As well as, DLL4 ligand binding to the NOTCH3 receptor mediates transendocytosis of NOTCH3-ADCs into ligand-expressing cells. NOTCH3-ADCs internalize into receptor and ligand cells impartial of signaling and induce cell demise in each cell sorts representing an atypical mechanism of ADC cytotoxicity. Remedy of xenografts with NOTCH3-ADCs results in sustained tumor regressions, outperforms standard-of-care chemotherapy, and permits concentrating on of tumors that overexpress NOTCH3 impartial of signaling inhibition.
Fc-binding antibody-recruiting molecules exploit endogenous antibodies for anti-tumor immune responses
Redirecting endogenous antibodies in the bloodstream to tumor cells utilizing artificial molecules is a promising method to set off anti-tumor immune responses. Nevertheless, present molecular designs solely allow using a small fraction of endogenous antibodies, limiting the therapeutic potential. Right here, we report Fc-binding antibody-recruiting molecules (Fc-ARMs) as the primary instance addressing this problem. Fc-ARMs are composed of an Fc-binding peptide and a concentrating on ligand, enabling the exploitation of endogenous antibodies by fixed affinity to the Fc area of antibodies, whose sequence is conserved in distinction to the Fab area.
We present that Fc-ARM concentrating on folate receptor-α (FR-α) redirects a clinically used antibody combination to FR-α+ most cancers cells, leading to most cancers cell lysis by pure killer cells in vitro. Fc-ARMs efficiently interacted with antibodies in vivo and gathered in tumors. Moreover, Fc-ARMs recruited antibodies to suppress tumor development in a mouse mannequin. Thus, Fc-ARMs have the potential to be a novel class of most cancers immunotherapeutic brokers.
Significance of hepatitis B floor antigen, IgM/IgG core antibody and hepatitis B virus DNA in blood donors
Introduction: Identification of hepatitis B virus carriers in blood donors is crucial with a view to keep away from transmission of the illness through blood transfusion.
Goal: To find out if blood donors with constructive outcomes for serological markers HBsAg and anti-HBc have been hepatitis B virus DNA carriers.
Strategies: 12,745 samples have been collected from six Ecuadorian blood banks and analyzed for HBsAg, anti-HBc and anti-HBs infectious markers by automated ELISA. All samples that examined constructive for one, two or all three markers have been analyzed with molecular strategies to find out the presence of viral DNA.
Outcomes: 27.5 % of the samples that have been reactive for anti-HBc alone and 100 % of these with constructive outcomes for HbsAg and IgM/IgG anti-HBc have been recognized to include hepatitis B virus DNA (p = 0.001).
Conclusions: The number of an infection markers, in addition to the detection strategies outline the outcomes. Performing two serological and one molecular check is necessary with a view to determine hepatitis B virus carriers and stop its transmission.
Introducción: La identificación de portadores del virus de la hepatitis B en donantes de sangre es imperativo para evitar la transmisión de la enfermedad a través de transfusiones sanguíneas.
Objetivo: Determinar si los donantes de sangre con resultados positivos en los marcadores serológicos HbsAg y anti-HBc eran portadores del ADN del virus de la hepatitis B.
Métodos: Se recolectaron 12 745 muestras de seis bancos de sangre ecuatorianos, las cuales fueron analizadas con pruebas serológicas para identificar marcadores infecciosos de HBsAg, anti-HBc, anti-HBs mediante ELISA automatizada. Todas las muestras positivas para uno, dos o los tres marcadores fueron analizadas con técnica molecular para determinar la presencia del ADN viral.
Resultados: Se identificó que 27.5 % de las muestras reactivas solo a anti-HBc y 100 % de las muestras con resultados positivos de HBsAg/anti-HBc-IgM/IgG presentaron ADN del virus de la hepatitis B (p = 0.001).
Conclusiones: La elección de los marcadores de infección y los métodos de detección definen los resultados. Es importante la realización de dos pruebas serológicas y una molecular para identificar a los portadores del virus de la hepatitis B y evitar su transmisión.
Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1
Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1
Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1
Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250
Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250
Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250
Description: Enzyme-linked immunosorbent assay kit for quantification of Human ALK-1/ACVRL1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Activin Receptor-Like Kinase 1 (ALK-1) is a type I cell-surface receptor for the TGF- beta superfamily of ligands, which mediates signaling of BMP9 (bone morphogenetic protein) and BMP10. ALK1 signaling is necessary for angiogenesis during embryogenesis, wound healing, and tumor growth. ALK-1 has a high degree of similarity in serine-threonine kinase subdomains, a glycine and serine rich region preceding the kinase-domain, and a C-terminal tail with other activin receptor-like kinase proteins. ALK-1 is mainly expressed in endothelial cells regulating proliferation and migration in vitro and angiogenesis in vivo. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting ALK-1 plays a critical role for in the control of blood vessel development or repair.
Description: Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ALK1 gene. ALK1 is a receptor in the TGF beta signaling pathway. ALK1 protein is a receptor in the TGF beta signaling pathway. It plays an important role in vascular development, remodeling, and pathologic angiogenesis, play a role in stabilizing angiogenic vessels and contribute to resistance to anti-VEGF therapies, ALK1 blockade may represent an effective therapeutic opportunity complementary to the current antiangiogenic modalities in the clinic. Recently, researcher found that, ALK1-Fc inhibited BMP9-mediated Id-1 expression in human umbilical vein endothelial cells and inhibited cord formation by these cells on a Matrigel substrate, in a chick chorioallantoic membrane assay, ALK1-Fc reduced vascular endothelial growth factor-, fibroblast growth factor-, and BMP10-mediated vessel formation, and ALK1-Fc treatment reduced tumor burden in mice receiving orthotopic grafts of MCF7 mammary adenocarcinoma cells.
Recombinant Human Activin Receptor-Like Kinase 1/ALK-1(C-Fc)
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Streptavidin.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is unconjugated.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 565.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 633.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 655.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 680.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to ATTO 700.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Alkaline Phosphatase.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to APC .
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to APC/Cy7.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Biotin.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Dylight 350.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Dylight 405.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Dylight 488.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Dylight 594.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Dylight 633.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to FITC.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to HRP.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to PE/ATTO 594.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to PerCP.
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to RPE .
Description: A polyclonal antibody for ALK (pTyr1507) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1507 of human ALK (AA374-382). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1507) antibody is conjugated to Streptavidin.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is unconjugated.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 565.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 633.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 655.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 680.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to ATTO 700.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Alkaline Phosphatase.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to APC .
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to APC/Cy7.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Biotin.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Dylight 350.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Dylight 405.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Dylight 488.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Dylight 594.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to Dylight 633.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to FITC.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to HRP.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to PE/ATTO 594.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to PerCP.
Description: A polyclonal antibody for ALK (pTyr1092) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Tyr1092 of human ALK (AA1504-1510). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ALK (pTyr1092) antibody is conjugated to RPE .
Mouse Anti Human Factor Viii Monoclonal Antibody,Alk. Phos.
Two human antibodies to a meningococcal serogroup B vaccine antigen improve binding of complement Issue H by stabilizing the Issue H binding web site
Microbial pathogens bind host complement regulatory proteins to evade the immune system. The bacterial pathogen Neisseria meningitidis, or meningococcus, binds a number of complement regulators, together with human Issue H (FH). FH binding protein (FHbp) is a element of two licensed meningococcal vaccines and in mice FHbp elicits antibodies that inhibit binding of FH to FHbp, which defeat the bacterial evasion mechanism. Nevertheless, people vaccinated with FHbp develop antibodies that improve binding of FH to the micro organism, which might restrict the effectiveness of the vaccines. Within the current research, we present that two vaccine-elicited antibody fragments (Fabs) remoted from totally different human topics enhance binding of complement FH to meningococcal FHbp by ELISA.
The 2 Fabs have totally different results on the kinetics of FH binding to immobilized FHbp as measured by floor plasmon resonance. The 1.7- and a couple of.0-Å decision X-ray crystal buildings of the Fabs in complexes with FHbp illustrate that the 2 Fabs bind to comparable epitopes on the amino-terminal area of FHbp, adjoining to the FH binding web site. Superposition fashions of ternary complexes of every Fab with FHbp and FH present that there’s seemingly minimal contact between the Fabs and FH. Collectively, the buildings reveal that the Fabs improve binding of FH to FHbp by altering the conformations and mobilities of two loops adjoining to the FH binding web site of FHbp. As well as, the 1.5 Å-resolution construction of one of many remoted Fabs defines the structural rearrangements related to binding to FHbp. The FH-enhancing human Fabs, that are mirrored within the human polyclonal antibody responses, have necessary implications for tuning the effectiveness of FHbp-based vaccines.
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [RefSeq]
Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody
Description: A sandwich ELISA kit for detection of Integrin Alpha 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Human Integrin Alpha 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Integrin Alpha 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Integrin Alpha 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Recombinant Antibody to Integrin Alpha 5 (ITGa5) (Human), Biotinylated
Description: A competitive ELISA for quantitative measurement of Human Integrin alpha-M in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Integrin alpha-M in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Integrin alpha-M in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
