Concanavalin A Lectin

  • Quality level: 200
  • Kind: Type VI
  • Form: lyophilized powder
  • Power: ≤ 20 μg per ml of agglutination activity
  • Impurities: Carbohydrate, essentially free
    salt, essentially free
  • Solubility: water: 1% (p / v)
  • Storage temperature: −20 ° C

Request

Concanavalin A from Canavalia ensiformis (Jack bean) has been used to develop zwitterion immobilized molecularly imprinted polymers (MIP) for protein crystallization. It has also been used in the preparation of protein-loaded microgels to prevent enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Packaging

  • 1 g in a polyethylene bottle
  • 25, 100, 250 mg in a polyethylene bottle

Biochemical / Physiological Actions

With A it is not blood group specific but has an affinity for the terminal α-D-mannosyl and α-D-glucosyl residues. Ca2 + and Mn2 + ions are necessary for activity. With A it dissociates into dimers at pH 5.6 or less. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. With A it exhibits mitogenic activity that depends on its degree of aggregation. Succinylation results in an active dimeric form that remains dimer above pH 5.6.

Preparation note

Highly purified by specific affinity chromatography.

Analysis note

Agglutination activity: determined from serial dilutions of a 1 mg/ml solution (this activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour of incubation at 25 ° C). Requires type A human blood.

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