Introduction

Mouse Interleukin-11 (IL-11) ELISA is a powerful immunoassay designed to detect and quantify IL-11, a cytokine involved in hematopoiesis, immune regulation, and inflammation. IL-11 plays a crucial role in bone metabolism, gastrointestinal homeostasis, and cancer progression. The Enzyme-Linked Immunosorbent Assay (ELISA) provides a sensitive, specific, and reproducible method for measuring IL-11 levels in various biological samples, including serum, plasma, and tissue homogenates. (nih.gov)

Key Features and Advantages

1. High Sensitivity and Specificity

The Mouse IL-11 ELISA is designed with high-affinity antibodies to ensure precise detection, even in low-abundance samples. It minimizes cross-reactivity with other cytokines, making it a reliable tool for research and diagnostics. (ncbi.nlm.nih.gov)

2. Quantitative and Reproducible Results

This assay offers a broad dynamic range and delivers consistent results across multiple runs, making it ideal for biomedical research and clinical studies. (genome.gov)

3. Rapid and Easy-to-Use Protocol

The assay follows a straightforward ELISA workflow, requiring minimal hands-on time and offering results in just 2–3 hours. It is compatible with standard 96-well microplates and spectrophotometers. (fda.gov)

4. Compatible with Multiple Sample Types

This ELISA kit can detect IL-11 in serum, plasma, cell culture supernatants, and tissue lysates, allowing for a wide range of research applications. (who.int)

5. Validated for Preclinical and Biomedical Research

IL-11 is implicated in several diseases, including osteoporosis, inflammatory bowel disease (IBD), and cancer, making this assay an essential tool for preclinical studies and biomarker discovery. (cancer.gov)

Applications in Research and Medicine

1. Inflammation and Immune Response Studies

IL-11 is involved in the regulation of immune responses and has been linked to chronic inflammatory diseases such as rheumatoid arthritis and colitis. Quantifying IL-11 can help researchers better understand its role in inflammation. (cdc.gov)

2. Bone Metabolism and Osteoporosis Research

IL-11 plays a role in osteoclast differentiation and bone remodeling, making it a valuable biomarker for studies on osteoporosis and skeletal diseases. (ncbi.nlm.nih.gov)

3. Cancer Research and Tumor Microenvironment Studies

Recent studies suggest that IL-11 contributes to tumor progression and metastasis, particularly in gastric and colorectal cancers. Measuring IL-11 can aid in developing novel cancer therapies. (cancer.gov)

4. Gastrointestinal Disease and Mucosal Healing

IL-11 has been found to support intestinal epithelial repair and regeneration, making it relevant in research on inflammatory bowel diseases (IBD) such as Crohn’s disease and ulcerative colitis. (genome.gov)

5. Cardiovascular Research

Studies indicate that IL-11 may be involved in cardiac fibrosis and heart failure, making it a potential therapeutic target for cardiovascular diseases. (heart.org)

Comparative Analysis: Mouse IL-11 ELISA vs. Other Cytokine Assays

Feature	Mouse IL-11 ELISA	Other Cytokine ELISA Kits
Specificity	High for IL-11	Varies by cytokine
Sensitivity	Detects pg/mL levels	Moderate to high
Dynamic Range	Broad (10-1000 pg/mL)	Moderate
Assay Time	2–3 hours	4–5 hours
Sample Compatibility	Serum, plasma, tissues	Some kits limited to serum/plasma

Compared to other cytokine assays, the Mouse IL-11 ELISA provides a highly specific, rapid, and versatile solution for researchers studying inflammation, oncology, and bone metabolism. (fda.gov)

Best Practices for Accurate Results

1. Sample Preparation and Storage

• Use fresh or properly stored (-80°C) serum/plasma samples to prevent cytokine degradation. (cdc.gov)
• Avoid repeated freeze-thaw cycles, which can affect cytokine stability. (who.int)

2. Proper Calibration and Controls

• Run a standard curve with each assay to ensure accurate quantification. (ncbi.nlm.nih.gov)
• Include positive and negative controls to monitor assay performance. (genome.gov)

3. Optimal Detection and Data Analysis

• Measure absorbance at 450 nm using a microplate reader. (fda.gov)
• Use appropriate software for curve fitting and statistical analysis. (nih.gov)

Conclusion

The Mouse Interleukin-11 ELISA is a highly sensitive and specific immunoassay for detecting IL-11 in biological samples. With its broad application range, ease of use, and rapid assay time, it is an essential tool for biomedical research, disease biomarker discovery, and therapeutic development.

By enabling precise quantification of IL-11 levels, this ELISA kit supports advancements in immunology, cancer research, osteoporosis studies, and inflammatory disease modeling. As interest in cytokine-targeted therapies and precision medicine grows, reliable assays like Mouse IL-11 ELISA will continue to play a pivotal role in translational and clinical research. (usgs.gov)